Identification of novel antioxidant peptides from snakehead (Channa argus) soup generated during gastrointestinal digestion and insights into the anti-oxidation mechanisms
نویسندگان
چکیده
Antioxidant peptides obtained from snakehead (Channa argus) soup (SHS) after simulated gastrointestinal (GI) digestion were separated, identified and characterized. Results showed that the fraction with MW < 3 kDa had highest antioxidant capacity. Four novel RP-HPLC UPLC-MS/MS. PGMLGGSPPGLLGGSPP SDGSNIHFPN DPPH radical scavenging activity (IC50 = 1.39 mM) Fe2+ chelating ability 4.60 mM), respectively. Structures in silico for IVLPDEGK, suggest at least one ?-turn and/or ?-helix, which are associated activity. Moreover, our results these three docked a recombinant Kelch-like ECH-associated protein 1 (Keap1) binding score greater than TX6, good ligand of Keap1. The cell viability assay also significant cytoprotective effects against H2O2-induced cellular oxidative damage. This information implies mechanisms SHS occurred via activation anti-oxidation Keap1-Nrf2 signaling pathway.
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ژورنال
عنوان ژورنال: Food Chemistry
سال: 2021
ISSN: ['1873-7072', '0308-8146']
DOI: https://doi.org/10.1016/j.foodchem.2020.127921